2OH6
The Crystal Structure of Recombinant Cypovirus Polyhedra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-05-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97885 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 102.955, 102.955, 102.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.500 - 2.100 |
| R-factor | 0.135 |
| Rwork | 0.129 |
| R-free | 0.19600 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.378 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.132 | 0.435 |
| Number of reflections | 10547 | |
| <I/σ(I)> | 8.2 | 2.6 |
| Completeness [%] | 97.9 | 98.2 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 300 | The crystals used to determine this structure were directly purified from cells, in vivo crystallization in the cytoplasm of the cell, temperature 300K |
