2OH3
Crystal structure of COG1633: Uncharacterized conserved protein (ZP_00055496.1) from Magnetospirillum magnetotacticum MS-1 at 2.00 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97913 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.360, 75.280, 89.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.110 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.105 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.035 | 0.365 |
| Number of reflections | 14778 | |
| <I/σ(I)> | 16.21 | 2.16 |
| Completeness [%] | 96.8 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 277 | NANODROP, 0.2M NaF, 20.0% PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.10 |
