2OGW
Structure of ABC type zinc transporter from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.974 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.900, 86.378, 87.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.660 - 1.830 |
| R-factor | 0.2254 |
| Rwork | 0.223 |
| R-free | 0.26860 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.660 | 1.878 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 47173 | |
| <I/σ(I)> | 16.68 | 3.99 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 10.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% PEG 3350, 200mM ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
