2OGI
Crystal structure of a putative metal dependent phosphohydrolase (sag1661) from streptococcus agalactiae serogroup v at 1.85 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97935, 0.97864 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.841, 52.535, 70.022 |
| Unit cell angles | 90.00, 114.29, 90.00 |
Refinement procedure
| Resolution | 40.550 - 1.850 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.22900 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.653 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.555 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.658 |
| Number of reflections | 37403 | |
| <I/σ(I)> | 11.13 | 2 |
| Completeness [%] | 99.6 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | NANODROP, 1.0M LiCl, 20.0% PEG-6000, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP, pH 6.00 |
