2OGG
Structure of B. subtilis trehalose repressor (TreR) effector binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97900 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 69.719, 69.719, 236.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.880 - 2.500 |
| R-factor | 0.19576 |
| Rwork | 0.190 |
| R-free | 0.24659 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.412 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.278 |
| Number of reflections | 10126 | |
| <I/σ(I)> | 50 | 3.2 |
| Completeness [%] | 95.6 | 70.5 |
| Redundancy | 6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Reservoir: 0.1M MES pH 6.0, 1.26M Ammonium sulfate, 0.56mM CYMAL-5. Protein: 21.2mg/ml. Drops: 1+1 microliter, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
