2OGG
Structure of B. subtilis trehalose repressor (TreR) effector binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-06 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97900 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 69.719, 69.719, 236.535 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.880 - 2.500 |
R-factor | 0.19576 |
Rwork | 0.190 |
R-free | 0.24659 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.412 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.060 | 0.278 |
Number of reflections | 10126 | |
<I/σ(I)> | 50 | 3.2 |
Completeness [%] | 95.6 | 70.5 |
Redundancy | 6 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Reservoir: 0.1M MES pH 6.0, 1.26M Ammonium sulfate, 0.56mM CYMAL-5. Protein: 21.2mg/ml. Drops: 1+1 microliter, VAPOR DIFFUSION, HANGING DROP, temperature 298K |