2OGF
Crystal structure of protein MJ0408 from Methanococcus jannaschii, Pfam DUF372
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.693, 46.270, 79.276 |
| Unit cell angles | 90.00, 118.54, 90.00 |
Refinement procedure
| Resolution | 27.000 - 1.890 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iec |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.446 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.066 | 0.337 |
| Number of reflections | 37996 | |
| <I/σ(I)> | 8.9 | 3 |
| Completeness [%] | 97.4 | 77.8 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 24% PEG-3350, Tris-HCL, 0.2 M Ammonium Sulfate, pH 4.6, Vapor diffusion, Sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
