2OFZ
Ultrahigh Resolution Crystal Structure of RNA Binding Domain of SARS Nucleopcapsid (N Protein) at 1.1 Angstrom Resolution in Monoclinic Form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.571, 36.440, 47.737 |
| Unit cell angles | 90.00, 94.71, 90.00 |
Refinement procedure
| Resolution | 47.570 - 1.170 |
| R-factor | 0.16389 |
| Rwork | 0.162 |
| R-free | 0.19132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | structure of homolog from infectious bronchitis virus PDB:2BTL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.373 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.570 | 1.200 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.062 | 0.497 |
| Number of reflections | 40016 | |
| <I/σ(I)> | 22.19 | 2.25 |
| Completeness [%] | 97.4 | 99.19 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 25% PEG 2000MME, 0.2 M sodium bromide, 0.1 M sodium acetate, bcip ADDITIVE, VAPOR DIFFUSION, SITTING DROP |
