2OFS
Crystal structure of human CD59
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-08 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 1.75 |
| Spacegroup name | P 63 |
| Unit cell lengths | 56.400, 56.400, 39.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.200 - 2.120 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cdq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.051 | 0.141 |
| Number of reflections | 4118 | |
| <I/σ(I)> | 52.9 | 12.5 |
| Completeness [%] | 99.3 | 93 |
| Redundancy | 12.2 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 294 | 0.1 M sodium citrate, 16 % polyethylene glycol 6000, 5% isopropanol, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
