2OFM
1.11 A Crystal Structure of Apo Nitrophorin 4 From Rhodnius Prolixus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.470, 42.816, 52.720 |
| Unit cell angles | 90.00, 94.71, 90.00 |
Refinement procedure
| Resolution | 17.400 - 1.110 |
| R-factor | 0.14803 |
| Rwork | 0.146 |
| R-free | 0.18266 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x80 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.664 |
| Data reduction software | MOSFLM (& scala) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.400 | |
| High resolution limit [Å] | 1.110 | 1.110 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 63130 | |
| <I/σ(I)> | 25 | 3.3 |
| Completeness [%] | 93.0 | 83 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
