2OEW
Structure of ALIX/AIP1 Bro1 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.678, 63.243, 76.384 |
| Unit cell angles | 90.00, 122.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| R-factor | 0.21167 |
| Rwork | 0.208 |
| R-free | 0.27386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zb1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.544 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.053 | 0.240 |
| Number of reflections | 15487 | |
| <I/σ(I)> | 19.6 | 3.6 |
| Completeness [%] | 96.5 | 79.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 286 | 10% PEG 20000, 0.1M NaMES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
