2OES
MSrecA-native-SSB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 103.672, 103.672, 72.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.930 - 3.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.930 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.130 | 0.542 |
| Number of reflections | 5789 | |
| <I/σ(I)> | 7.8 | 1.5 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Citrate Phosphate, Sodium Citrate, PEG 4000, NACL, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
