2OER
Probable Transcriptional Regulator from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.255, 46.389, 56.978 |
| Unit cell angles | 100.62, 101.49, 109.84 |
Refinement procedure
| Resolution | 27.620 - 2.000 |
| R-factor | 0.21984 |
| Rwork | 0.212 |
| R-free | 0.29322 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.620 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.399 |
| Number of reflections | 25222 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 95.1 | 90 |
| Redundancy | 4.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 25% PEG3350, 0.2M MgCl2, 0.1M Bis-Tris pH 5.5, 2% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
