2OEO
Cryogenic crystal structure of Staphylococcal Nuclease variant truncated Delta+PHS I92D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.452, 57.630, 34.618 |
| Unit cell angles | 90.00, 99.74, 90.00 |
Refinement procedure
| Resolution | 20.790 - 2.000 |
| R-factor | 0.1961 |
| Rwork | 0.196 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1snc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.790 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.042 | 0.136 |
| Number of reflections | 8029 | |
| <I/σ(I)> | 10.1 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20-35% MPD, 10% glycerol, 5% DMSO, 10.5 mM potassium phosphate in the presence of Ca2+ and pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
