2OEK
Crystal structure of a rubisco-like protein from Geobacillus kaustophilus liganded with Mg2+ ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.765, 59.545, 109.282 |
| Unit cell angles | 90.00, 103.24, 90.00 |
Refinement procedure
| Resolution | 24.830 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 20EJ |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.044 |
| Number of reflections | 74695 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 24%peg 3350, 0.1 M tris-HCl, 0.2 M ammonium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
