2OEG
Open and Closed Structures of the UDP-Glucose Pyrophosphorylase from Leishmania major
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97920, 0.97960, 0.950 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.247, 89.929, 137.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21001 | |
| Completeness [%] | 96.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 27% PEG monomethyl ether 2000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
