2OEB
The crystal structure of gene product Af1862 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 73.309, 73.309, 147.192 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.320 - 1.660 |
| R-factor | 0.18755 |
| Rwork | 0.185 |
| R-free | 0.23358 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.362 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.320 | 1.703 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.055 | 0.531 |
| Number of reflections | 16823 | |
| <I/σ(I)> | 39.02 | 2.53 |
| Completeness [%] | 96.8 | 93.23 |
| Redundancy | 9.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2M Ammonium Acetate, 0.1M Na Citate, 30% PEG 2K MME, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
