2ODV
Crystal structure of a fragment of the plakin domain of plectin, Cys to Ala mutant.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9785, 0.9185 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 154.620, 26.510, 58.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.660 - 2.050 |
| R-factor | 0.21227 |
| Rwork | 0.210 |
| R-free | 0.25599 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.150 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 15444 | |
| <I/σ(I)> | 31.7 | 6 |
| Completeness [%] | 97.7 | 99.6 |
| Redundancy | 14.1 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M Citrate Phosphate pH 4.6, 24% 1,2-propanediol, 6% PEG 3000, 3% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
