2ODU
Crystal structure of a fragment of the plakin domain of plectin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9785, 0.9185 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 154.150, 26.340, 58.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.470 - 2.300 |
| R-factor | 0.21873 |
| Rwork | 0.216 |
| R-free | 0.26930 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 11241 | |
| <I/σ(I)> | 22.4 | 4.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M Citrate Phosphate pH 4.6, 10% 1,2-propanediol, 5% PEG 3000, 4% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
