2ODQ
Complement component C2a, the catalytic fragment of C3- and C5-convertase of human complement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.354, 84.201, 74.426 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrk |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.313 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.047 | 0.180 |
| Number of reflections | 27904 | |
| <I/σ(I)> | 19.5 | 7.1 |
| Completeness [%] | 98.8 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 20% PEG 10000, 0.1M HEPES, 0.3M glycyl-glycyl-glycine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
