2ODH
Restriction Endonuclease BCNI in the Absence of DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.176, 68.176, 127.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.620 - 1.600 |
R-factor | 0.204 |
Rwork | 0.203 |
R-free | 0.21500 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.342 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXD |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 40038 | |
<I/σ(I)> | 4 | 3.1 |
Completeness [%] | 99.2 | 99.4 |
Redundancy | 5.8 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 0.17 M ammonium acetate, 0.085 M tri-sodium citrate pH 5.6, 25.5% PEG 4000, 15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |