2ODF
The crystal structure of gene product Atu2144 from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.523, 89.765, 120.365 |
| Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
| Resolution | 120.000 - 1.900 |
| R-factor | 0.19113 |
| Rwork | 0.189 |
| R-free | 0.23394 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.358 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 1.952 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 | 0.420 |
| Number of reflections | 173247 | |
| <I/σ(I)> | 22 | 2.3 |
| Completeness [%] | 99.0 | 95.72 |
| Redundancy | 4.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 5% Isopropanol, 2M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
