2OD6

Crystal structure of a dimeric ferredoxin-like protein (jcvi_pep_1096682647733) from uncultured marine organism at 1.85 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97944, 0.97972
Spacegroup name	P 21 21 21
Unit cell lengths	36.433, 92.842, 144.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.086 - 1.850
R-factor	0.198
Rwork	0.197
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.681
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.086	29.090	1.900
High resolution limit [Å]	1.800	8.270	1.850
Rmerge	0.072	0.042	0.611
Total number of observations		1402	11297
Number of reflections	42961
<I/σ(I)>	6.1	14.9	1.2
Completeness [%]	99.8	86.1	100
Redundancy	3.6	2.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	9	277	30.0% PEG-6000, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K