2OD0
The crystal structure of gene product VP1028 from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.458, 42.876, 157.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.060 - 1.950 |
| R-factor | 0.20187 |
| Rwork | 0.200 |
| R-free | 0.24521 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.108 | 0.494 |
| Number of reflections | 20605 | |
| <I/σ(I)> | 20 | 2.1 |
| Completeness [%] | 99.4 | 97.16 |
| Redundancy | 8.3 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 2 M Ammonium sulfate, 0.1 M Citrate buffer, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
