2OCU
Structure of the complex of C-terminal lobe of bovine lactoferrin with N-(4-hydroxyphenyl) acetamide at 2.38 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54132 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.216, 50.398, 65.838 |
| Unit cell angles | 90.00, 107.60, 90.00 |
Refinement procedure
| Resolution | 63.250 - 2.380 |
| R-factor | 0.17382 |
| Rwork | 0.157 |
| R-free | 0.22257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2B6D |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.619 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 2.460 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 14913 | |
| Completeness [%] | 97.8 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | HEPES, PEG Monomethyl Ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
