Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.180, 120.030, 154.350 |
| Unit cell angles | 90.00, 90.68, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j90 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.112 | 0.318 |
| Number of reflections | 61311 | |
| <I/σ(I)> | 8.2 | 2.2 |
| Completeness [%] | 97.5 | 95.8 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | PEG 4000, AMMONIUM ACETATE, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
