2OCJ

Human p53 core domain in the absence of DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-24
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	68.913, 69.364, 84.179
Unit cell angles	90.00, 90.11, 90.00

Refinement procedure

Resolution	50.000 - 2.050
Rwork	0.209
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tsr chain B
RMSD bond length	0.006
RMSD bond angle	1.268
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.162	0.535
Number of reflections	49562
<I/σ(I)>	26.4	6.7
Completeness [%]	99.1	98.4
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	2 ul protein solution (5 mg/ml protein in 20 mM Tris, pH 7.6, 150 mM NaCl, 10 mM DTT) were mixed with 2 ul reservoir buffer consisting of 100 mM Hepes, pH 7.6, 10 mM DTT, and 14-21% (w/v) polyethylene glycol (PEG) 4000 above 500 ul reservoir buffer. Colorless plate-shaped crystals were obtained within a few days. VAPOR DIFFUSION, SITTING DROP, temperature 293K