2OC5

Crystal structure of a ferritin-like protein (pmt1231) from prochlorococcus marinus str. mit 9313 at 1.68 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97929, 0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	77.350, 77.350, 116.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.235 - 1.680
R-factor	0.166
Rwork	0.164
R-free	0.20000
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.472
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.235		1.740
High resolution limit [Å]	1.680	2.870	1.680
Rmerge	0.069	0.057	0.545
Number of reflections	41123
<I/σ(I)>	10.19	19.2	2.08
Completeness [%]	99.6	99.8	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	7.8	277	0.2M KAcetate, 20.0% PEG-3350, No Buffer pH 7.8, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K