2OBM
Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5428 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.131, 78.690, 53.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.21088 |
| Rwork | 0.208 |
| R-free | 0.26329 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1obl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.060 | 0.379 |
| Number of reflections | 17196 | |
| <I/σ(I)> | 36.3 | 5.23 |
| Completeness [%] | 98.8 | 97.1 |
| Redundancy | 8.6 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 7% (w/v) PEG 8000, 0.1M Calcium Acetate, 0.1M Imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
