2OBH
Centrin-XPC peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-11-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.278, 59.419, 105.137 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 52.400 - 1.800 |
| R-factor | 0.205 |
| Rwork | 0.201 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1top |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.405 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.400 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.395 |
| Number of reflections | 34221 | |
| <I/σ(I)> | 17.4 | 1.8 |
| Completeness [%] | 99.3 | 95.5 |
| Redundancy | 5.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 30% MPEG 550, 0.1M NaCl, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
