2OB0
Human MAK3 homolog in complex with Acetyl-CoA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97931, 0.97945, 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.523, 103.324, 67.328 |
| Unit cell angles | 90.00, 106.79, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20400 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.518 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 56481 | |
| <I/σ(I)> | 28.8 | 2 |
| Completeness [%] | 98.2 | 88.5 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | Reservoir: 0.1 M Sodium acetate pH 5.0, 16% PEG3350, 10 mM DTT, 0.1% Dioxane. Protein: 11.8 mg/mL + 3-fold molar excess of ACOA in 20 mM Hepes pH 7.5, 0.1 M NaCl, 2.5 % 1,2-propandiol, and 10 mM DTT. Cryo: 75% reservoir + 25% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |
