2OAA
Restriction endonuclease MvaI-cognate DNA substrate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.848, 81.380, 71.308 |
| Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oa9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.189 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 94979 | |
| <I/σ(I)> | 6.2 | 2.5 |
| Completeness [%] | 96.6 | 95.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 294 | 0.1 M HEPES pH 7.89, 0.2 M CaCl2, 25 % PEG4000, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | PEG4000 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | CaCl2 | ||
| 6 | 1 | 2 | PEG4000 | ||
| 7 | 1 | 2 | H2O |
