2OA9
Restriction endonuclease MvaI in the absence of DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.499, 63.281, 71.892 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.500 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.525 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 75246 | |
| <I/σ(I)> | 30.8 | 4.1 |
| Completeness [%] | 91.2 | 99.6 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 294 | 0.05 M CADMIUM SULFATE HYDRATE, 0.1 M HEPES PH 7.74, 1.2 M SODIUM ACETATE TRIHYDRATE, 0.17 M GLYCINE, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 7.70 |
