2O9T
beta-glucosidase B from Bacillus polymyxa complexed with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | Nonius Kappa CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.330, 75.580, 88.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.330 - 2.150 |
| Rwork | 0.245 |
| R-free | 0.27500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2o9p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.313 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.048 | 0.038 | 0.288 |
| Number of reflections | 26625 | ||
| <I/σ(I)> | 25.4 | ||
| Completeness [%] | 99.3 | 98.1 | 97.4 |
| Redundancy | 3.4 | 5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 30% PEG550MME, 0.05M Cl2Ca, 0.1M bis/tris, pH 6.5, VAPOR DIFFUSION |
