2O9R
beta-glucosidase B complexed with thiocellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | KAPPA 200 CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.144, 75.394, 88.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.260 - 2.300 |
| Rwork | 0.201 |
| R-free | 0.27300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2o9p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.339 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.230 | 2.310 |
| Rmerge | 0.056 | 0.450 |
| Number of reflections | 23404 | |
| <I/σ(I)> | 20 | 1.9 |
| Completeness [%] | 98.1 | 95.1 |
| Redundancy | 4.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 30% PEG 550MME, 0.05M Cl2Ca, 0.1M bis-tris, pH 6.5, VAPOR DIFFUSION |
