2O9P
beta-glucosidase B from Paenibacillus polymyxa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.895, 74.958, 88.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.235 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e4i |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.100 |
| Rmerge | 0.088 | 0.480 |
| Number of reflections | 32444 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 30% PEG 550 MME, 0.05M Cl2Ca, 0,1M bis-tris, pH 6.5, VAPOR DIFFUSION |
