2O9B
Crystal Structure of Bacteriophytochrome chromophore binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-27 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.500, 51.500, 80.800 |
| Unit cell angles | 90.00, 116.40, 90.00 |
Refinement procedure
| Resolution | 23.710 - 2.150 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.23695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ZTU without chromophore or his tag |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.806 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.104 | 0.333 |
| Number of reflections | 17484 | |
| <I/σ(I)> | 10 | 2.7 |
| Completeness [%] | 96.4 | 78.8 |
| Redundancy | 6.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.095 M sodium citrate, 19% v/v isopropanol, 19% v/v PEG 4000, 5% V/V glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
