2O9A
The crystal structure of the E.coli IclR C-terminal fragment in complex with pyruvate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-16 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.499, 81.434, 154.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.010 - 1.800 |
| R-factor | 0.17607 |
| Rwork | 0.173 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.806 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.010 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.036 | 0.161 |
| Number of reflections | 62028 | |
| <I/σ(I)> | 21.6 | 5.9 |
| Completeness [%] | 97.8 | 85 |
| Redundancy | 4.03 | 2.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 0.2M Potassium Acetate pH 7.0, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
