2O98
Structure of the 14-3-3 / H+-ATPase plant complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-14 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 114.370, 114.370, 236.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.18247 |
| Rwork | 0.178 |
| R-free | 0.26276 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.140 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.079 | 0.461 |
| Number of reflections | 21892 | |
| <I/σ(I)> | 16.48 | 4.05 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.53 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | VAPOR DIFFUSION, HANGING DROP, temperature 277K |
