2O97
Crystal Structure of E. coli HU heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-20 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 41 |
| Unit cell lengths | 82.915, 82.915, 61.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.035 | 0.362 |
| Number of reflections | 7084 | |
| <I/σ(I)> | 28.3 | |
| Completeness [%] | 92.8 | 64.7 |
| Redundancy | 6.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 0.1M Tris-HCl, 0.01M nickel chloride, 20% PEG-MME2000, 5% glycerol, pH 8.5, EVAPORATION, temperature 298K, pH 8.50 |
