2O96
Crystal Structure of the Metal-Free Dimeric Human Mov34 MPN domain (residues 1-177)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.701, 96.010, 60.982 |
| Unit cell angles | 90.00, 122.14, 90.00 |
Refinement procedure
| Resolution | 51.640 - 3.000 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o95 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.590 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.278 | 51.650 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.088 | 0.027 | 0.562 |
| Total number of observations | 1025 | 4562 | |
| Number of reflections | 10578 | ||
| <I/σ(I)> | 7.9 | 19.8 | 1.3 |
| Completeness [%] | 93.9 | 86.9 | 96.3 |
| Redundancy | 3 | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M citrate, 0,2M NH4OAc, 30% PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
