2O8P
Crystal structure of a putative 14-3-3 protein from Cryptosporidium parvum, cgd7_2470
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 87.325, 87.325, 167.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.820 |
| R-factor | 0.19894 |
| Rwork | 0.198 |
| R-free | 0.23911 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.105 | 0.859 |
| Number of reflections | 28702 | |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 98.1 | 87.1 |
| Redundancy | 9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 297 | 15% PEG 3350, 0.3 M ammonium acetate, 0.2 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
