2O84

Crystal structure of K206E mutant of N-lobe human transferrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2005-06-07
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	44.173, 57.317, 135.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.020 - 2.600
R-factor	0.216
Rwork	0.213
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	N-lobe human transferrin PDB code 1A8E
RMSD bond length	0.010
RMSD bond angle	1.273
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.3.0008)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.700	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.139	0.377
Number of reflections	10917
<I/σ(I)>	9	3
Completeness [%]	98.5	100
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	Protein: 35mg/ml, 0.1M ammonium bicrbonate. Well: 18% PEG3350, 0.2M potassium acetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K