2O7U
Crystal structure of K206E/K296E mutant of the N-terminal half molecule of human transferrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.462, 97.897, 208.953 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 29.760 - 2.800 |
| R-factor | 0.23109 |
| Rwork | 0.230 |
| R-free | 0.25885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Wild type transferrin. PDB entry code 1A8E. |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.323 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 | 0.406 |
| Number of reflections | 83306 | |
| <I/σ(I)> | 16.4 | 3.2 |
| Completeness [%] | 98.5 | 98.8 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | Protein: 35mg/ml, 0.1M ammonium bicarbonate. Well: 20% PEG 3350, 0.2 M potassium acetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
