2O7U
Crystal structure of K206E/K296E mutant of the N-terminal half molecule of human transferrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-15 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.462, 97.897, 208.953 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 29.760 - 2.800 |
R-factor | 0.23109 |
Rwork | 0.230 |
R-free | 0.25885 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Wild type transferrin. PDB entry code 1A8E. |
RMSD bond length | 0.011 |
RMSD bond angle | 1.323 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.074 | 0.406 |
Number of reflections | 83306 | |
<I/σ(I)> | 16.4 | 3.2 |
Completeness [%] | 98.5 | 98.8 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | Protein: 35mg/ml, 0.1M ammonium bicarbonate. Well: 20% PEG 3350, 0.2 M potassium acetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |