2O7T

Crystal structure of a tetr family transcriptional regulator (ncgl1578, cgl1640) from corynebacterium glutamicum at 2.10 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.92522, 0.97975
Spacegroup name	P 32 2 1
Unit cell lengths	51.197, 51.197, 129.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.162 - 2.100
R-factor	0.183
Rwork	0.180
R-free	0.23000
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.423
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.162	26.160	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.083	0.034	0.686
Total number of observations		669	3768
Number of reflections	12118
<I/σ(I)>	6.1	18.4	1.1
Completeness [%]	99.8	93.5	98.9
Redundancy	5.1	4.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	8.5	277	15.0% Glycerol, 0.17M NaOAc, 25.5% PEG-4000, 0.1M TRIS pH 8.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K