2O7P
The crystal structure of RibD from Escherichia coli in complex with the oxidised NADP+ cofactor in the active site of the reductase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2836 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 173.000, 173.000, 77.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.930 - 3.000 |
| R-factor | 0.21496 |
| Rwork | 0.212 |
| R-free | 0.26653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g6v |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.784 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.084 | 0.506 |
| Number of reflections | 27042 | |
| <I/σ(I)> | 15.6 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) at pH 7.4, 150mM NaCl, 10mM mercaptoethanol, 10% (v/v) glycerol, 2mM EDTA, 0.1M MES pH 6.5, 3% (v/v) 1,6 Hexandiol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
