2O7G
Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Collection date | 2004-12-22 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 85.173, 85.173, 79.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.460 - 2.700 |
| R-factor | 0.20348 |
| Rwork | 0.201 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1or7 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.763 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.010 | 2.890 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 9076 | |
| <I/σ(I)> | 10.9 | 2.3 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 2M ammonium sulphate, 5% isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
