2O7G

Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Collection date	2004-12-22
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	85.173, 85.173, 79.554
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.460 - 2.700
R-factor	0.20348
Rwork	0.201
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1or7
RMSD bond length	0.030
RMSD bond angle	2.763
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0009)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.010	2.890
High resolution limit [Å]	2.700	2.700
Number of reflections	9076
<I/σ(I)>	10.9	2.3
Completeness [%]	99.8	99.2
Redundancy	2.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	2M ammonium sulphate, 5% isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K