2O7C

Crystal structure of L-methionine-lyase from Pseudomonas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-10-10
Detector	RIGAKU RAXIS V
Wavelength(s)	0.8340
Spacegroup name	P 43 21 2
Unit cell lengths	133.129, 133.129, 215.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.700
Rwork	0.204
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.525
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.700
Rmerge	0.075
Number of reflections	173038
Completeness [%]	98.0
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	20% PEG 6000, 0.1M ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K