2O7A
T4 lysozyme C-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.634, 50.017, 32.711 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.840 |
| R-factor | 0.0904 |
| Rwork | 0.090 |
| R-free | 0.10780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sx7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.037 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.870 |
| High resolution limit [Å] | 0.840 | 0.840 |
| Rmerge | 0.071 | 0.224 |
| Number of reflections | 92458 | |
| <I/σ(I)> | 31.8 | 2.5 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 100 mM NaCacodylate pH 5.0, 200 mM NaAcetate, 26% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
