2O68
Crystal Structure of Haemophilus influenzae Q58L mutant FbpA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-04 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.771, 75.736, 33.953 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.020 - 1.700 |
R-factor | 0.19791 |
Rwork | 0.195 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d9v |
RMSD bond length | 0.008 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.760 | 1.830 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 27533 | |
Completeness [%] | 89.0 | 89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 550 MME, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |