2O66
Crystal Structure of Arabidopsis thaliana PII bound to citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-14 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.709, 66.752, 61.743 |
| Unit cell angles | 90.00, 118.93, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.19278 |
| Rwork | 0.191 |
| R-free | 0.22406 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ul3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.244 |
| Number of reflections | 25717 | |
| <I/σ(I)> | 24 | 5 |
| Completeness [%] | 99.2 | 95.3 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.4 M ammonium citrate, 10% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
